Fig. 1. RBC necroptosis leads to Syk-dependent post-transfusion clearance. A.) Human CD59 transgenic murine RBCs were treated with sub-lytic quantities of VLY or PLY ex vivo for 30 min followed by transfusion into healthy, recipient WT mice. VLY, which induces RBC necroptosis, induces RBC clearance while PLY does not. B.) VLY-induced clearance is not affected by nec-1s. Shown is the ratio of vehicle-treated to nec-1s-treated RBCs. C.) VLY-induced clearance is prevented by inhibition of Syk kinase. Shown is the ratio of toxin-treated:untreated RBCs. Results shown are from 10 transfused mice in each experiment. Mann-Whitney, ***p<0.001.